Protein solubility kits are designed to help you in identifying the formulation that will protect your target proteins from aggregation. Solubility screening kits are also used to solubilize aggregated protein samples. The results you get from your tests will largely depend on the choice you make on the kit.
Some of the uses for the kit include quick aggregation behavior based on PH, salt and the presence of additives. It also assesses numerous conditions in a label free and parallel form. The processes require simple mixing and spinning without the use of centrifuge. This simplifies your tasks and allows you to get desired results in whichever condition.
The results obtained using the screening kits enable you to rescue reversibly aggregated protein samples. Your proteins will also not aggregate under stress. This makes them available for different tests and functions. You are provided with a dashboard with trends that make it easy to interpret results from the tests. The dashboard makes it easier to relate the results and make inferences.
The process of protein aggregation is undesirable because it hinders certain functions. Some of the conditions that lead to aggregation include addition of ligands, protein binding partners and elevated temperatures. Prolonged storage and vigorous stirring also cause aggregation. When the right decision on salt, PH and stabilizing additives is made, aggregation can be avoided.
The testing kit comes with buffers of PH that ranges from three to ten. You will also get solubility enhancers like polyols, amino acids, salts, reducing agents and sugars. These enhancers give precise results on the conditions in which your proteins will aggregate or de-aggregate.
Working with this kit allows you to get over ninety formulations using a single label free experiment. You will use the other six as control to get positive and negative assessments. The expected results include soluble proteins going through the filters while aggregated ones do not. The filtration process will help you get these results.
The tests using the protein solubilization kit help you to establish the conditions under which your target proteins will remain in solution form. If a well contains soluble proteins during a test, the filtration process will yield proteins. There will be no traces of proteins if they aggregate during the experiment.
The choice of conditions is determined by the results of screening. Since you can identify the conditions that facilitate the right behavior and the proteins in question, you can make an informed decision. It is used to confirm if the target substances are right for the expected results.
The benefits of using screening kits over other testing methods are numerous. Results obtained are clear and easy to interpret based on the information available in the field. The results leave no room for doubt. It takes as little as one hour to get your results. These results are certain and not based on trial and error.
The approach substantially reduces protein consumption during experiments. Because of this reduction, it is easy to match the target proteins with the assay. A detailed report is received allowing you to make many more inferences and conclusions. The kits do not restrict the assay that may be used during testing.
Some of the uses for the kit include quick aggregation behavior based on PH, salt and the presence of additives. It also assesses numerous conditions in a label free and parallel form. The processes require simple mixing and spinning without the use of centrifuge. This simplifies your tasks and allows you to get desired results in whichever condition.
The results obtained using the screening kits enable you to rescue reversibly aggregated protein samples. Your proteins will also not aggregate under stress. This makes them available for different tests and functions. You are provided with a dashboard with trends that make it easy to interpret results from the tests. The dashboard makes it easier to relate the results and make inferences.
The process of protein aggregation is undesirable because it hinders certain functions. Some of the conditions that lead to aggregation include addition of ligands, protein binding partners and elevated temperatures. Prolonged storage and vigorous stirring also cause aggregation. When the right decision on salt, PH and stabilizing additives is made, aggregation can be avoided.
The testing kit comes with buffers of PH that ranges from three to ten. You will also get solubility enhancers like polyols, amino acids, salts, reducing agents and sugars. These enhancers give precise results on the conditions in which your proteins will aggregate or de-aggregate.
Working with this kit allows you to get over ninety formulations using a single label free experiment. You will use the other six as control to get positive and negative assessments. The expected results include soluble proteins going through the filters while aggregated ones do not. The filtration process will help you get these results.
The tests using the protein solubilization kit help you to establish the conditions under which your target proteins will remain in solution form. If a well contains soluble proteins during a test, the filtration process will yield proteins. There will be no traces of proteins if they aggregate during the experiment.
The choice of conditions is determined by the results of screening. Since you can identify the conditions that facilitate the right behavior and the proteins in question, you can make an informed decision. It is used to confirm if the target substances are right for the expected results.
The benefits of using screening kits over other testing methods are numerous. Results obtained are clear and easy to interpret based on the information available in the field. The results leave no room for doubt. It takes as little as one hour to get your results. These results are certain and not based on trial and error.
The approach substantially reduces protein consumption during experiments. Because of this reduction, it is easy to match the target proteins with the assay. A detailed report is received allowing you to make many more inferences and conclusions. The kits do not restrict the assay that may be used during testing.
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